primary antibodies rabbit anti-human ifnar1 and ifnar2 gtx54289  (GeneTex)

Journal: The Journal of Reproduction and Development

Article Title: Pregnancy-associated changes of peroxisome proliferator-activated receptor delta (PPARD) and cytochrome P450 family 21 subfamily A member 2 (CYP21A2) expression in the bovine corpus luteum

doi: 10.1262/jrd.2019-132

Figure Lengend Snippet: Primers used in real-time PCR

Article Snippet: The 5 µm-thick sections from the paraffin-embedded luteal tissue were incubated at room temperature (20˚C) with rabbit polyclonal anti-human PPARD antibody (#ARP38765_T100, Aviva Systems Biology, San Diego, CA, USA; 1:50), rabbit polyclonal anti-human CYP21A2 antibody (#ARP60144_P050, Aviva Systems Biology; 1:25), rabbit polyclonal anti-human IFNαR1 antibody (#bs-4116R, Bioss, Woburn, MA, USA; 1:50) or rabbit polyclonal anti-human IFNαR2 antibody (#bs-7022R, Bioss; 1:50) for 12 h. The signals were detected using anti-rabbit IgG-Biotin conjugate (Sigma-Aldrich, LLC, St Louis, MO, USA; 1:100) for 1 h, and then counterstained with hematoxylin.

Techniques: Sequencing

Journal: The Journal of Reproduction and Development

Article Title: Pregnancy-associated changes of peroxisome proliferator-activated receptor delta (PPARD) and cytochrome P450 family 21 subfamily A member 2 (CYP21A2) expression in the bovine corpus luteum

doi: 10.1262/jrd.2019-132

Figure Lengend Snippet: Changes in the relative amount of mRNA for IFNαR1 and IFNαR2 in the bovine corpus luteum (CL) on day 18 of pregnant cows (P18) and on day 15 of non-pregnant cows (NP15). Data represent means ± SEM for four cows per stage and have been expressed as relative ratios of the mRNAs to 18S ribosomal RNA ( RN18S1 ). Localization of IFNαR1 and IFNαR2 in bovine CL obtained on P18 and NP15. Intensive immunoreactivity was observed in the luteal cells (black arrowheads), endothelial cells (red arrowheads), and immune cells (yellow arrowheads) obtained from the CL of both pregnant and nonpregnant cows. No positive immunoreactivity was observed in the negative control. Scale bar = 50 µm.

Article Snippet: The 5 µm-thick sections from the paraffin-embedded luteal tissue were incubated at room temperature (20˚C) with rabbit polyclonal anti-human PPARD antibody (#ARP38765_T100, Aviva Systems Biology, San Diego, CA, USA; 1:50), rabbit polyclonal anti-human CYP21A2 antibody (#ARP60144_P050, Aviva Systems Biology; 1:25), rabbit polyclonal anti-human IFNαR1 antibody (#bs-4116R, Bioss, Woburn, MA, USA; 1:50) or rabbit polyclonal anti-human IFNαR2 antibody (#bs-7022R, Bioss; 1:50) for 12 h. The signals were detected using anti-rabbit IgG-Biotin conjugate (Sigma-Aldrich, LLC, St Louis, MO, USA; 1:100) for 1 h, and then counterstained with hematoxylin.

Techniques: Negative Control

Journal: Respiratory Research

Article Title: Expression of human Interferon Regulatory Factor 3 (IRF-3) in alveolar macrophages relates to clinical and functional traits in COPD.

doi: 10.1186/s12931-024-02952-6

Figure Lengend Snippet: Main immunohistochemical findings in the study groups

Article Snippet: Briefly, formalin-fixed paraffin embedded lung sections were treated for 60 min with primary antibodies rabbit anti-human IFNAR1 and IFNAR2 (GTX54322 and GTX54289 from GeneTex, Irvine CA, USA), MDA-5 (700360 from Invitrogen, Carlsbad CA, USA) and murine anti-human IRF-3 (sc-376455 from Santa Cruz Dallas TX, USA).

Techniques: Immunohistochemical staining

Journal: Respiratory Research

Article Title: Expression of human Interferon Regulatory Factor 3 (IRF-3) in alveolar macrophages relates to clinical and functional traits in COPD.

doi: 10.1186/s12931-024-02952-6

Figure Lengend Snippet: Additional immunohistochemical findings (intensity score in alveolar walls and bronchiolar epithelium)

Article Snippet: Briefly, formalin-fixed paraffin embedded lung sections were treated for 60 min with primary antibodies rabbit anti-human IFNAR1 and IFNAR2 (GTX54322 and GTX54289 from GeneTex, Irvine CA, USA), MDA-5 (700360 from Invitrogen, Carlsbad CA, USA) and murine anti-human IRF-3 (sc-376455 from Santa Cruz Dallas TX, USA).

Techniques: Immunohistochemical staining

Journal: Respiratory Research

Article Title: Expression of human Interferon Regulatory Factor 3 (IRF-3) in alveolar macrophages relates to clinical and functional traits in COPD.

doi: 10.1186/s12931-024-02952-6

Figure Lengend Snippet: Main immunohistochemical findings in the study groups

Article Snippet: Briefly, formalin-fixed paraffin embedded lung sections were treated for 60 min with primary antibodies rabbit anti-human IFNAR1 and IFNAR2 (GTX54322 and GTX54289 from GeneTex, Irvine CA, USA), MDA-5 (700360 from Invitrogen, Carlsbad CA, USA) and murine anti-human IRF-3 (sc-376455 from Santa Cruz Dallas TX, USA).

Techniques: Immunohistochemical staining

Journal: Respiratory Research

Article Title: Expression of human Interferon Regulatory Factor 3 (IRF-3) in alveolar macrophages relates to clinical and functional traits in COPD.

doi: 10.1186/s12931-024-02952-6

Figure Lengend Snippet: Additional immunohistochemical findings (intensity score in alveolar walls and bronchiolar epithelium)

Article Snippet: Briefly, formalin-fixed paraffin embedded lung sections were treated for 60 min with primary antibodies rabbit anti-human IFNAR1 and IFNAR2 (GTX54322 and GTX54289 from GeneTex, Irvine CA, USA), MDA-5 (700360 from Invitrogen, Carlsbad CA, USA) and murine anti-human IRF-3 (sc-376455 from Santa Cruz Dallas TX, USA).

Techniques: Immunohistochemical staining